Journal of Global Infectious Diseases

ORIGINAL ARTICLE
Year
: 2013  |  Volume : 5  |  Issue : 2  |  Page : 49--53

Multiplex PCR for rapid diagnosis of gastrointestinal tuberculosis


Kusum Sharma1, Saroj Kant Sinha2, Aman Sharma3, Ritambra Nada4, Kaushal K Prasad2, Kapil Goyal1, Surinder Singh Rana2, Deepak Kumar Bhasin2, Meera Sharma1 
1 Department of Medical Microbiology, Post Graduate Institute of Medical Education and Research, Chandigarh, India
2 Department of Gastroenterology, Post Graduate Institute of Medical Education and Research, Chandigarh, India
3 Department of Internal Medicine, Post Graduate Institute of Medical Education and Research, Chandigarh, India
4 Department of Pathology, Post Graduate Institute of Medical Education and Research, Chandigarh, India

Correspondence Address:
Kusum Sharma
Department of Medical Microbiology, Post Graduate Institute of Medical Education and Research, Chandigarh
India

Background: Rapid and specific diagnosis of gastrointestinal tuberculosis (GITB) is of utmost importance. Aim: To evaluate Multiplex PCR (MPCR) using MPB64 and IS6110 primers specific for M. tuberculosis for rapid diagnosis of GITB. Materials and Methods: MPCR was performed on colonoscopy biopsy specimens on 11 GITB confirmed (culture/AFB/histopathology was positive), 29 GITB suspected and 30 Non GITB (control group) patients. Results: MPB64 PCR had sensitivity and specificity of 90% and 100% for confirmed GITB cases. In 29 clinically diagnosed but unconfirmed GITB cases, MPCR was positive in 72.41%. MPCR was negative in all control group patients. The overall sensitivity and specificity of microscopy, culture, histopathology and MPCR was 5%, 2% 20% and 77.5% and 100%, 100%, 100% and 100% respectively. Conclusion: MPCR has good sensitivity and specificity in diagnosing gastrointestinal tuberculosis.


How to cite this article:
Sharma K, Sinha SK, Sharma A, Nada R, Prasad KK, Goyal K, Rana SS, Bhasin DK, Sharma M. Multiplex PCR for rapid diagnosis of gastrointestinal tuberculosis.J Global Infect Dis 2013;5:49-53


How to cite this URL:
Sharma K, Sinha SK, Sharma A, Nada R, Prasad KK, Goyal K, Rana SS, Bhasin DK, Sharma M. Multiplex PCR for rapid diagnosis of gastrointestinal tuberculosis. J Global Infect Dis [serial online] 2013 [cited 2021 Dec 2 ];5:49-53
Available from: https://www.jgid.org/article.asp?issn=0974-777X;year=2013;volume=5;issue=2;spage=49;epage=53;aulast=Sharma;type=0