LETTER TO EDITOR
Year : 2013 | Volume
: 5 | Issue : 2 | Page : 86--87
Diagnosis of extrapulmonary tuberculosis by polymerase chain reaction for mpb64 gene: An evaluation in a hospital based study
Sushma Pednekar1, Arvind V Bhore2, Vrishali A Muley2, Dnyaneshwari P Ghadage2,
1 Department of Microbiology, B. J. Medical College and Sassoon, General Hospitals, Pune, India
2 Smt. Kashibai Navale Medical College and General Hospitals, Mumbai-Pune, Bypass, Pune, India
Vrishali A Muley
Smt. Kashibai Navale Medical College and General Hospitals, Mumbai-Pune, Bypass, Pune
|How to cite this article:|
Pednekar S, Bhore AV, Muley VA, Ghadage DP. Diagnosis of extrapulmonary tuberculosis by polymerase chain reaction for mpb64 gene: An evaluation in a hospital based study.J Global Infect Dis 2013;5:86-87
|How to cite this URL:|
Pednekar S, Bhore AV, Muley VA, Ghadage DP. Diagnosis of extrapulmonary tuberculosis by polymerase chain reaction for mpb64 gene: An evaluation in a hospital based study. J Global Infect Dis [serial online] 2013 [cited 2021 Dec 4 ];5:86-87
Available from: https://www.jgid.org/text.asp?2013/5/2/86/112283
Extrapulmonary tuberculosis (EPTB) often possesses a diagnostic dilemma. Microscopy and culture have proved to be insensitive techniques for diagnosis of EPTB.  The present study was conducted to evaluate the role of polymerase chain reaction (PCR) in the early diagnosis of clinically suspected cases of EPTB.
A total of 50 clinical specimens comprising pleural fluid, cerebrospinal fluid, ascitic fluid, fine needle aspiration biopsy, pus and biopsy from clinically suspected EPTB cases were processed and followed by conventional methods , and PCR using MPB64 primer.
Tuberculous pleural effusion (68%) was found to be the commonest clinical presentation of EPTB. Overall, PCR could detect EPTB in 62% cases. Microscopy and culture could detect 16% and 20% EPTB cases respectively. PCR was positive in all the tissue samples suggestive of tuberculosis on histopathological examination. Of the 39 EPTB patients who responded to antituberculosis treatment, 31 patients were PCR positive.
PCR using MPB64 had a significant advantage over the conventional methods in diagnosing EPTB cases.
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