Journal of Global Infectious Diseases

ORIGINAL ARTICLE
Year
: 2018  |  Volume : 10  |  Issue : 4  |  Page : 196--200

Comparative proteomic profiling of Mycobacterium tuberculosis and the Thai vaccine strain Mycobacterium bovis Bacille Calmette–Guerin Tokyo172: Diverse biomarker candidates for species differentiation


Songsri Kasempimolporn, Pornpimol Premchaiporn, Wichit Thaveekarn, Supatsorn Boonchang, Visith Sitprija 
 Department of Research and Development, Queen Saovabha Memorial Institute, Thai Red Cross Society, Bangkok 10330, Thailand

Correspondence Address:
Dr. Songsri Kasempimolporn
Department of Research and Development, Queen Saovabha Memorial Institute, Thai Red Cross Society, Bangkok 10330
Thailand

Background: Bacille Calmette–Guerin (BCG)-related complications can occur in vaccinated children. Comparison of the composition of cellular proteins of virulent Mycobacterium tuberculosis (MTB) H37Rv with of attenuated Mycobacterium bovis BCG Tokyo172 vaccine strain used in Thailand and identify protein candidates of value for differentiation between the two mycobacterial species may facilitate the diagnosis of etiologic agent of mycobacterial disease in vaccinated children, as most cases have been believed to have originated from BCG vaccine. Materials and Methods: The two-dimensional electrophoresis (2DE) proteomic profiles of cellular proteins from the Thai vaccine strain M. bovis BCG Tokyo172 and MTB were compared and the matched spots in 2DE gels were submitted to mass spectrometry analysis. Results: There were a number of similar protein contents with different intensity or position between MTB and M. bovis BCG Tokyo172. A higher expression of some immunogenic proteins was shown in BGG Tokyo172 when compared to MTB, while some were shown the opposite pattern. Conclusions: Proteomic approach reveals key proteins participating in different species of Mycobacteria, and may be useful for discrimination between MTB and the BCG Tokyo172 infection.


How to cite this article:
Kasempimolporn S, Premchaiporn P, Thaveekarn W, Boonchang S, Sitprija V. Comparative proteomic profiling of Mycobacterium tuberculosis and the Thai vaccine strain Mycobacterium bovis Bacille Calmette–Guerin Tokyo172: Diverse biomarker candidates for species differentiation.J Global Infect Dis 2018;10:196-200


How to cite this URL:
Kasempimolporn S, Premchaiporn P, Thaveekarn W, Boonchang S, Sitprija V. Comparative proteomic profiling of Mycobacterium tuberculosis and the Thai vaccine strain Mycobacterium bovis Bacille Calmette–Guerin Tokyo172: Diverse biomarker candidates for species differentiation. J Global Infect Dis [serial online] 2018 [cited 2022 Sep 27 ];10:196-200
Available from: https://www.jgid.org/article.asp?issn=0974-777X;year=2018;volume=10;issue=4;spage=196;epage=200;aulast=Kasempimolporn;type=0